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More Proof Coronavirus
Genome Manipulated Pre-SARS

From Patricia Doyle, PhD
dr_p_doyle@hotmail.com>
4-21-3

Hello Jeff - The US research actually wanted to prove that the coronavirus could be genetically manipulated. Bear in mind that the virus chosen was MOUSE HEPATITIS, one which was dominant in the SARS agent genetic material. It is quite possible that the victims of SARS may develop an MS LIKE syndrome later on (that is, if I am correct).
 
This is developing and I hope to have more research for you to PROVE that the SARS agent was being manipulated long before the outbreak.
 
Patty
 
 
From: Curr Opin Microbiol 2001 Aug;4(4):462-6 Related Articles, Links
 
 
Mouse hepatitis virus.
 
Haring J, Perlman S.
 
Departments of Microbiology and Pediatrics, University of Iowa, Medical Laboratories 2042, Iowa City, IA 52242, USA. jodie-haring@uiowa.edu
 
Inoculation of mice with most neurotropic strains of the coronavirus mouse hepatitis virus results in an immune response-mediated demyelinating disease that serves as an excellent animal model for the human disease multiple sclerosis. Recent work has shown that either virus-specific CD4(+) or CD8(+) T cells are able to mediate demyelination and also that the antibody response is crucial for clearing infectious virus. Another exciting advance is the development of recombinant coronaviruses, which, for the first time, will allow genetic manipulation of the entire viral genome.
 
Publication Types:
Review
Review, Tutorial
 
PMID: 11495812 [PubMed - indexed for MEDLINE]
 
Location:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=R
etrieve&db=PubMed&list_uids=11495812&dopt=Abstract
 
 
 
PS - Now here is proof of scientists playing God with viruses:
 
 
Excerpt:
"This work demonstrates for the first time the feasibility of incorporating foreign amino acid sequences (up to 18 residues) into a protein component of the ASFV particle without affecting virus viability."
 
Arch Virol 1999;144(7):1287-98 Related Articles, Links
 
Design and construction of African swine fever virus chimeras incorporating foreign viral epitopes.
 
Brun A, Rodriguez F, Parra F, Sobrino F, Escribano JM.
 
Centro de Investigacion en Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, Spain.
 
In the present work we have studied the feasibility of introducing foreign epitopes into the African swine fever virus (ASFV) particle by genetic manipulation of the virus. For this purpose, we developed specific transfer vectors containing the gene encoding for the highly antigenic structural ASFV protein p54 in which foreign sequences were introduced. DNA sequences encoding continuous linear epitopes, the antigenic site A from foot-and-mouth disease virus (FMDV) VP1 protein and the DA3 antigenic determinant from transmissible gastroenteritis coronavirus (TGEV) nucleoprotein N, were separately cloned into the p54 gene, in a region encoding a non-essential domain of the protein. Chimeric p54 genes were inserted by homologous recombination into the thymidine kinase (TK) locus of ASFV genome. The resulting recombinant viruses efficiently expressed both chimeric proteins under transcriptional control of the p54 promoter, and the chimeric gene products were recognized by antibodies to both p54 and foreign epitopes. The modified p54 proteins were also found in the viral particles and complemented the function of the wild-type p54, since deletion of the p54 gene from recombinant viruses did not affected virus replication in Vero cells. This work demonstrates for the first time the feasibility of incorporating foreign amino acid sequences (up to 18 residues) into a protein component of the ASFV particle without affecting virus viability.
 
PMID: 10481737 [PubMed - indexed for MEDLINE]
 
 
Patricia A. Doyle, PhD
Please visit my "Emerging Diseases" message board at:
http://www.clickitnews.com/emergingdiseases/index.shtml
Zhan le Devlesa tai sastimasa
Go with God and in Good Health


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