- Hello Jeff - The US research actually wanted to prove
that the coronavirus could be genetically manipulated. Bear in mind that
the virus chosen was MOUSE HEPATITIS, one which was dominant in the SARS
agent genetic material. It is quite possible that the victims of SARS
may develop an MS LIKE syndrome later on (that is, if I am correct).
-
- This is developing and I hope to have more research for
you to PROVE that the SARS agent was being manipulated long before the
outbreak.
-
- Patty
-
-
- From: Curr Opin Microbiol 2001 Aug;4(4):462-6 Related
Articles, Links
-
-
- Mouse hepatitis virus.
-
- Haring J, Perlman S.
-
- Departments of Microbiology and Pediatrics, University
of Iowa, Medical Laboratories 2042, Iowa City, IA 52242, USA. jodie-haring@uiowa.edu
-
- Inoculation of mice with most neurotropic strains of
the coronavirus mouse hepatitis virus results in an immune response-mediated
demyelinating disease that serves as an excellent animal model for the
human disease multiple sclerosis. Recent work has shown that either virus-specific
CD4(+) or CD8(+) T cells are able to mediate demyelination and also that
the antibody response is crucial for clearing infectious virus. Another
exciting advance is the development of recombinant coronaviruses, which,
for the first time, will allow genetic manipulation of the entire viral
genome.
-
- Publication Types:
- Review
- Review, Tutorial
-
- PMID: 11495812 [PubMed - indexed for MEDLINE]
-
- Location:
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=R
- etrieve&db=PubMed&list_uids=11495812&dopt=Abstract
-
-
-
- PS - Now here is proof of scientists playing God with
viruses:
-
-
- Excerpt:
- "This work demonstrates for the first time the feasibility
of incorporating foreign amino acid sequences (up to 18 residues) into
a protein component of the ASFV particle without affecting virus viability."
-
- Arch Virol 1999;144(7):1287-98 Related Articles, Links
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- Design and construction of African swine fever virus
chimeras incorporating foreign viral epitopes.
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- Brun A, Rodriguez F, Parra F, Sobrino F, Escribano JM.
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- Centro de Investigacion en Sanidad Animal (CISA-INIA),
Valdeolmos, Madrid, Spain.
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- In the present work we have studied the feasibility of
introducing foreign epitopes into the African swine fever virus (ASFV)
particle by genetic manipulation of the virus. For this purpose, we developed
specific transfer vectors containing the gene encoding for the highly antigenic
structural ASFV protein p54 in which foreign sequences were introduced.
DNA sequences encoding continuous linear epitopes, the antigenic site A
from foot-and-mouth disease virus (FMDV) VP1 protein and the DA3 antigenic
determinant from transmissible gastroenteritis coronavirus (TGEV) nucleoprotein
N, were separately cloned into the p54 gene, in a region encoding a non-essential
domain of the protein. Chimeric p54 genes were inserted by homologous recombination
into the thymidine kinase (TK) locus of ASFV genome. The resulting recombinant
viruses efficiently expressed both chimeric proteins under transcriptional
control of the p54 promoter, and the chimeric gene products were recognized
by antibodies to both p54 and foreign epitopes. The modified p54 proteins
were also found in the viral particles and complemented the function of
the wild-type p54, since deletion of the p54 gene from recombinant viruses
did not affected virus replication in Vero cells. This work demonstrates
for the first time the feasibility of incorporating foreign amino acid
sequences (up to 18 residues) into a protein component of the ASFV particle
without affecting virus viability.
-
- PMID: 10481737 [PubMed - indexed for MEDLINE]
-
-
- Patricia A. Doyle, PhD
- Please visit my "Emerging Diseases" message
board at:
- http://www.clickitnews.com/emergingdiseases/index.shtml
- Zhan le Devlesa tai sastimasa
- Go with God and in Good Health
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