- Note - Donald Scott, in his 1-25, 3 hour interview with
Jeff, made the case that mycoplasma fermentans incognitus, a deadly human-engineered
killer, is responsible from a number of catastrophic diseases. Here is
a small excerpt from the US Army patent on Mycoplasma Incognitus Fermentens.
We urge you to listen to the Scott interview and to order the large packet
of printed scientific, clinical research material supporting his contention.
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- Tapes of the Scott interview can be ordered for only
$12 by calling 888 836-4670.
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- For the printed material, send $20 to:
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- Donald Scott
Box 133
Stn B Sudbury, Ontario Canada
P3E 4N5
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- Here are quoted sections from the following US patent
demonstrating the totality of infection with M. fermentans incognitus in
AIDS victims. Is M. incognitus an 'opportunistic' infection - or causal
factor of AIDS?
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- From US Patent -
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- LINK
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- RESULTS
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- Thymus
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- Many patients with AIDS suffer a profound deficiency
in cell mediated immunity. It is well known that development of competent
T-cell immunity is thymus dependent. Therefore, four thymic tissues available
from patients with AIDS were examined for possible M. fermentans incognitus
infection. Two of the thymic tissues were described grossly at autopsy
as involuted thymus, one from a two year old and the other from a eight
year old. Both of these pediatric patients contracted AIDS from blood transfusions.
The other two thymuses were derived from adult AIDS patients and the autopsy
reports contained no specific gross tissue description. Immunohisto-chemical
studies, using M. fermentans incognitus-specific monoclonal antibodies,
showed positive immunoreaction in all four thymic tissues. Both mononuclear
lymphohistiocytes and epitheloid cells were stained positively (FIG. 26).
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- FIG. 26 shows the immunhoistochemistry of thymic tissues
derived from patients with AIDS. FIG. 26A is a low-magnification photograph
of a thymus immunostained by M. fermentans incognitus-specific monoclonal
antibody (C42H10) (X71.5). FIG. 26B is a higher magnification of the positively
immunostained lymphohistiocytes in the junction between cortex and medulla
shown in 26A, left curve arrow (X715). FIG. 26C is a higher magnification
of the positively immunostained lymphohistiocytes in the septal interstitial
tissues in 26A, right curve arrow (X715). FIG. 26D is a low-magnification
photograph of a thymus from another AIDS patient (X126.5). FIG. 26E is
a higher magnification of the positively immunostained cells in 26D (X142).
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- Electron microscopic examination of the areas of the
thymus with significant positive immunoreaction showed ultrastructurally
many particles resembling mycoplasma. The particles were located both intracellularly
in the cytoplasm of lymphohistiocytes (FIG. 27 A, B) and apparently free-growing
extracellularly (FIG. 27 C, D). FIG. 27 shows an electron micrograph of
an AIDS thymus immunostained positively for M. fermentans incognitus-specific
antigens. FIG. 27A is an electron micrograph of mononuclear lymphohistiocytes
with many intracytoplasmic electron dense mycoplasma-like particles (arrows)
(N is the nucleus and bar represents 100 nm). FIG. 27B is a higher magnification
of the electron dense mycoplaslma-like particles in the cytoplasm of a
mononuclear cell shown in 27A (P is a polysomal structure and bar represents
100 nm). FIG. 27C is an electron micrograph of many mycoplasma-like particles
found both inside the membrane bound cytoplasmic vesicle (arrow heads)
and also extracellularly in the interstitial tissue (arrows) (N is the
nucleus with degenerating changes, Bar represents 100 nm). FIG. 27D is
a higher magnification of the extracellular mycoplasma-like particles.
The outer limiting membrane of some particles (arrows) can be identified
(Bar represents 100 nm). Most of the nearly spherical particles measured
100-300 nm. No cell wall was associated with these particles. However,
a prominent halo with a clear space surrounding each of these intracellular
particles was commonly noted.
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- Occasional cells exhibited cytopathological changes and
even appeared to be necrotic. However, most cells in these tissues were
morphologically unremarkable. There was no tissues reactive process and/or
an inflammatory reaction identified.
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- Liver
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- Ten livers from patients with AIDS who had unexplained
abnormal liver function tests were examined. Work-ups for both hepatitis
B and A infections were negative in these patients.
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- Four of these ten livers were positive by immunohistochemistry
using M. fermentans incognitus-specific monoclonal antibodies. Histopathology
of these four livers varied from no pathological changes except mild periportal
infiltrates of lymphohistiocytes (two) to fulminant hepatocyte necrosis
without any inflammatory reaction (one) and patchy areas of hepatocyte
necrosis associated with prominent acute and subacute inflammation (one).
The positively immunostained cells in these livers were the infiltrating
inflammatory cells and the hepatocytes with or without any evidence of
cytopathological changes (FIG. 28). Some areas of sinusoidal space lined
by Kupffer cells were also stained positively.
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- FIG. 28 shows the immunohistochemistry of livers derived
from patients with AIDS, using monoclonal antibody C42H10. FIG. 28A is
a photomicrograph at a portal area in an AIDS liver with patchy areas of
necrosis. Prominent infiltrates of chronic inflammatory cells and proliferation
of bile ducts (arrows) are identified (X390). FIG. 28B is a higher magnification
of the positively immunostained cells in 28A (X780).
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- FIG. 28C is the same portal area shown in 28A in a subsequent
tissue section immunostained by a nonspecific monoclonal antibody with
the same isotype IgCl/k. Hemosiderin pigments (arrow heads) are noted (X390).
FIG. 28D is an immunohistochemical photomicrograph of another AIDS liver.
No necrosis or histopathological changes other than mild infiltrates of
chronic inflammatory cells in the portal area (P) can be found in the liver
(X390).
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- The areas of liver showing positive M. fermentans incognitus-
specific antigens were also retrieved from the original paraffin blocks
for ultra structural examination. Microorganisms with typical mycoplasma
morphology were identified in all four livers. These mycoplasma-like microorganisms
could be found intracellularly in the cytoplasms of mononuclear lymphohistiocytes,
Kupffer cells and hepatocytes. Many of these microorganisms also lined
up extracellularly along the walls of sinusoids (FIG. 29). For comparison,
an electron micrograph of M. fermentans incognitus in the liver of a silvered
leaf monkey, experimentally infected with this pathogen (Example 9) is
shown in the insert of FIG. 29E.
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- FIG. 29 shows an electron micrograph of AIDS liver immunostained
positively for M. fermentans incognitus-specific antigens. FIG. 29A is
an electron micrograph of a periportal area of an AIDS liver with adjacent
necrosis. N is the nucleus of a mononuclear lymphohistiocyte. R is red
blood cells in the small vessel and the bar represents 500 nm. FIG. 29B
is a higher magnification of the mycoplasma-like microorganisms found in
the empty extracellular space and lining along the outer surface of the
lymphohistiocyte shown in 29A. Many intracellular particles (arrow heads)
can also be identified and are difficult to differentiate with the extracellular
particles (P is the polysomal structure and the bar represents 1200 nm).
FIG. 29C is a higher magnification of the mycoplasma-like microorganisms
lining the outer surface of the lymphohistiocyte (Bar represents 100 nm).
FIG. 29D is an electron micrograph of another AIDS liver which showed no
evidence of histopathological changes except mild portal infiltrates of
chronic inflammatory cells (N is the nucleus and the bar represents 400
nm). FIG. 33E is a higher magnification of the mycoplasma-like particles
shown in 29D. The insert shows M. fermentans incognitus in 2% glutaldehyde
fixed liver of experimentally infected silvered leaf monkey at the same
magnification (Bar represents 100 nm).
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- Lymph node and spleen
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- Two lymph nodes surgically removed from AIDS patients
showed reactive changes with follicular hyperplasia and foci of sinus histiocytosis.
No areas of necrosis were identified. Positive immunochemical reactions
were seen primarily within the endothelial cells lining the lymphatic sinus
or the mononuclear lymphohistiocytes found in the sinus. Both nuclei and
cytoplasm were stained positively. The typical staining patterns were similar
to the results presented previously, using polyclonal rabbit antiserum
(Lo, S-C et al., Am. J. Trop. Med. Hyg. 40, 213 (1989)).
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- Sections from four of six autopsy spleens without pathological
changes stained positively with M. fermentans incognitus-specific monoclonalantibody.
Mononuclear histiocytes and reticular cells in periarterial regions, mononuclear,
reticular cells and lymphocytes in areas of red pulps were the positive
cells which often revealed varying degrees of swelling or disruption. The
strongly-stained nuclei and cytoplasm resembled inclusion bodies in the
immunochemical reaction. Positively stained cells could also be identified
in two additional splenic tissues with areas of prominent necrosis. The
positive immunochemical reaction was concentrated at periphery of the necrosis
(data not shown).
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- Characteristic ultrastructures with morphological features
typical of mycoplasma were identified in all four spleens (including two
with necrosis) and two lymph nodes which were retrieved for electron microscopy.
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- Brain
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- More than 60% of patients with AIDS are reported to have
abnormal central nervous system (CNS) symptoms (Navaia, B. A. et al., Ann.
Neurol. 19, 517 (1986)). Since most AIDS patients have serological evidence
of HIV infection, the CNS diseases in these patients with AIDS have been
called HIV encephalopathy.
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- Eight brains from patients with AIDS who had prominent
clinical symptoms of CNS diseases without histopathological diagnosis of
a specific infection in the brains at necropsy were examined. Two of these
8 brains had lesions of fulminant necrosis and karyorrhexis associated
with both acute and subacute inflammations. Both of these brains were from
intravenous drug abusers with AIDS. One of the other brains had subacute
encephalitis with mononuclear cell infiltration but no necrosis. The remaining
5 brains showed only atrophy, gliosis and occasional microglial nodules
without evidence of necrosis or inflammation.
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- All 3 brains with histopathological evidence of acute
or subacute encephalitis stained positively for M. fermentans incognitus-specific
antigens. FIG. 30 shows the positive immunostaining of the acute and subacute
inflammatory cells in the periphery of a necrotic brain lesion.
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- FIG. 30A is a photomicrograph of the periphery of a necrotic
cerebellar lesion immunostained positively by M. fermentans incognitus-specific
monoclonal antibody (C42H10) (X390). FIG. 30B is a higher magnification
of the periphery of the lesion in 30A and shows both acute and subacute
inflammatory cells immunostained positively (X780). FIG. 30C is also a
higher magnification of the positively stained cells in 30A (X780). FIG.
30D is a photomicrograph of the same periphery area of the necrotic lesion
immunostained by a non-specific monoclonal antibody with the same isotype
IgG1/k. Cells with prominent cytopathological changes and disruption (arrows)
are evident (X780).
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- Furthermore, three of the 5 brains showing no evidence
of inflammation or necrosis also revealed positive immunostaining. The
positively stained cells showed degenerating changes, and often became
inclusion body-like structures in the gray and white matter. The patterns
and characteristics of positive immunohistochemical staining identified
in these histologically unremarkable brains were comparable to those previously
reported, using rabbit polyclonal antiserum (Lo, S-C et al., Am. J. Trop.
Med. Hyg. 40, 213 (1989)).
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- Ultrastructural confirmation of M. fermentans incognitus
infection in these 6 brains which immunostained positively for M. fermentans
incognitus-specific antigens was also performed. Many electron-dense particles
with features of mycoplasma organisms were identified extracellularly positively
for M. fermentans incognitus-specific antigens was also performed. Many
electron-dense particles with features of mycoplasma organisms were identified
extracellularly or in the cytoplasm of mononuclear lymphohistiocytes located
in the periphery of necrosis. Clusters of particles with morphological
features of mycoplasma could also be identified in the encephalopathy AIDS
brains showing positive immunostaining but with no evidence of necrosis
and inflammation (FIG. 31). Some of the particles had prominent outer membranes.
For comparison, the electron micrograph of M. fermentans incognitus with
an apparent outer limiting membrane identified in cytoplasm of Sb51 cells
in culture is shown in the insert of FIG. 31D.
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- FIG. 31A is an electron micrograph of mycoplasma-like
particles (arrows) clustered together in the hippocampus. F is a bundle
of neuroglialfilament and N is the nucleus of a mononuclear cell (Bar represents
100 nm). FIG. 31B is a higher magnification of the mycoplasma-like particles
shown in 31A. The outer limiting membrane (small arrows) of some particles
is prominent. (Bar represents 100 nm).
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- FIG. 31C is a higher magnification of the same particles.
FIG. 31D is a high magnification electron micrograph of mycoplasma-like
particles found in the brain stem from another AIDS patient (large photo
to right). The typical particles with well-preserved outer membrane (small
arrows) are shown in an endothelial cell. Cytoplasmic membrane (large arrows)
of the endothelial cells and basement membrane (arrow heads) of the vessel
can be identified. L is the lumen of the vessel. The insert shows an electron
micrograph of VLIA (M. fermentans incognitus) originally identified in
the cytoplasm of sb51 cells, at the same magnification. The unit membrane
of M. fermentans incognitus (small arrows) is prominent in the well fixed
(2% glutaldehyde) and well preserved culture specimen. Cytoplasmic membrane
(large arrows) of the sb51 cell is also identified (Bar represents 200
nm).
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- Placentas
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- Two placentas delivered at full term by two women with
AIDS were available for study. The babies were reported to be normal at
birth. However, no follow-up was available.
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- Histopathological examination showed occasional infiltrate
of acute inflammatory cells in the chorionic plates in one of the placentas.
The second placenta was histologically unremarkable. The special histopathological
stains did not reveal any pathogens in either of the two placentas. Immunohistochemical
study of both placentas, using M. fermentans incognitus-specific monoclonal
antibodies C42H10 and D81E7, exhibited positive immunoreaction in areas
of Hofbauer cells and stomal connective tissues in the chorionic villi
(FIG. 32). Some decidual cells in the stratum basalis were also stained
positively.
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- FIG. 32 shows the immunohistochemistry of a placenta
delivered by a patient with AIDS. FIG. 32A is a photomicrograph of placenta
tissue positively immunostained by a M. fermentans incognitus-specific
monoclonal antibody (C42H10). The insert shows the same placental area
in a subsequent tissue section immunostained by a non-specific monoclonal
antibody with the same isotype IgG1/k (X 195). FIG. 32B is a higher magnification
of the positively immunostained cells shown in 32A. The cytoplasm (arrow
heads) or the surface of vacuolated cells (arrows) more often reveals positive
reaction. Cells showing cytopathological changes with both nuclei and cytoplasms
are positively stained (curve arrows) may resemble atypical inclusion bodies
(X780).
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- Electron microscopic examination of the Hofbauer cells
and connective tissues in the positively stained chorionic villi revealed
numerous particles characteristic of mycoplasma (FIG. 33). Some particles
identified in the Hofbauer cells were probably in membrane bound vesicles.Many
microorganisms, with a wide variation of size, shape and electron density,
appeared to focally colonize in the stomal connective tissue (FIG. 33).
A prominent halo with a clear space surrounding each of these particles
was often noted. No accompanying acute inflammatory cells or other reactive
process was identified. Some apparently better preserved particles exhibited
recognizable outer limiting membranes. However, many of the mycoplasma-like
particles did not have definite outer unit membranes; they showed only
an electron dense internal matrix with a fine granular configuration.
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- FIG. 33 shows electron microscopy of an AIDS patient's
placenta immunostained a positively for M. fermentans incognitus specific
antigens.FIG. 33A is an electron micrograph of a Hofbauer cell containing
may mycoplasma-like particles in the cytoplasm. Some particles are apparently
in the membrane bound cytoplasmic vesicles (arrows). N is the nucleus and
I is a cytoplasmic inclusion body (Bar represents 800 nm). FIG. 33B is
a higher magnification of the mycoplasma-like particles. Both spherical
electron dense particles (arrow heads) and flask shape particles (arrows)
typical for mycoplasma organisms are found to colonize in the stomal connective
tissue (Bar represents 1000 nm). FIG. 33D is a higher magnification of
the mycoplasma-like particles shown in 33C. Typical electron dense internal
matrix with fine granular configuration of these particles is shown. Occasional
particles contain recognizable outer membrane (arrows) (bar represents
100 nm). FIG. 33E shows many of the particles are also those of less electron
dense but with granular appearing internal matrix. These particles often
have more prominent outer limiting membrane (arrows) (Bar represents 100
nm).
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- Detection of M. fermentans incognitus specific genetic
material
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- M. fermentans incognitus DNA was identified in the tissues
of thymus, liver and spleen from patients with AIDS as well as in the placentas
delivered by two women with AIDS using the .sup.35 S labeled psb-2.2 probe.
FIG. 34 shows positive labeling with grains heavily concentrated in cells
of livers and spleen. Cytological and/or histological identification of
the specific "types" of cells containing M. fermentans incognitus
DNA, revealed that they were the Kupffer cells and hepatocytes in the liver
showing minimal histopathological changes (FIG. 34A), the infiltrating
lymphoid cells and histiocytes in portal tracts of another liver (FIG.
34C), and the lymphocytes in periarteriolar lymphoid sheaths (white pulp)
of spleen (FIG. 34D).
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- In parallel, .sup.35 S-labeled M13 mp 19 vector DNA which
did not contain M. fermentans incognitus DNA, did not elicit any positive
signals in the consecutive sections from these tissues (FIG. 34B). Five
tissues of spleen and liver from three patients who died of non-AIDS conditions
were used as negative controls and also did not reveal any evidence of
positive signals.
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- FIG. 34 shows in situ hybridization for M. fermentans
incognitus nucleic acid in liver and spleen from patients with AIDS. FIG.
34A shows cells with strong labeling (arrows) are seen in an AIDS liver
with no histopathological abnormally after hybridization with .sup.35 S
labeled psb-2.2 DNA. Higher magnification (insert) reveals dense clusters
of grains over individual hepatocytes or Kupffer cells (X240, X770). FIG.
34B is the same area of 34A in the consecutive tissue section, hybridized
with .sup.35 S-labelled cloning vector DNA not containing M. fermentans
incognitus DNA (X270). FIG. 34C shows lymphocytes and histiocytes with
positive labeling seen in the portal tract infiltrated with mononuclear
inflammatory cells in the liver of another AIDS patient (X770). FIG. 34D
shows lymphocytes with strong labeling seen in the periarteriolar lymphoid
sheath of the spleen. The central arteriole (Ar) is identified. The insert
shows higher magnification of heavily concentrated grains over the lymphoid
cells in this white pulp (X350, X770).
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- Kidney
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- Renal tissues from 203 patients who died of AIDS as defined
by the Centers for Disease Control criteria were selected for study. The
patients lived in various geographic locations including the continental
United States (US), Puerto Rico (PR), Haiti, and Africa. The different
racial backgrounds included in this study were white, black, Hispanic,
and Oriental. Risk activities for AIDS were varied and included intravenous
drug abuse (IVDA), homosexual contact, heterosexual contact, and history
of blood transfusion. The patients had a wide range of opportunistic infectious
agent including Pneumocystis carinii, Toxoplasma gondii, Candida albicans,
Cryptococcus neoformans, Histoplasma capsulatum, Mycobacterium avium-intracellulare,
M tuberculosis, cytomegalovirus, herpes simplex virus, and others. Of the
203 total patients comprising this study, 20 patients had renal histopathologic
changes characteristic of AIDS-associated nephropathy (AAN). Group B consisted
of 15 patients selected from the remaining 183 who had no significant clinical
or pathologic evidence of renal disease. These patients were matched as
closely as possible with Group A patients in terms of the distribution
of age, gender, race, and risk activities which Sections of kidney from
the autopsies of 203 patients with AIDS, as well as renal tissues from
the five (Group C) controls, were examined by conventional light microscopy.
Special stains, including periodic acid-Schiff, Grocott's methenamine silver,
Ziehl-Neelsen, mucicarmine, Masson's trichrome, and Brown and Hopps, were
obtained to evaluate glomerular and tubular morphology as well as to document
the presence of various opportunistic infections. For the 20 cases of AAN,
glomerular, tubular, and interstitial changes were semiquantitatively graded
and recorded. Renal tissues from 15 of the 20 patients from Group A and
all of the tissues from Groups B and C were evaluated using monoclonal
antibodies (MABs) against M. fermentans incognitus as described above.
Formalin-fixed, paraffin-embedded sections of kidney were immunochemically
stained with MABs against the incognitus strain, as previously described.
Specific areas of positive staining were circled (approximately 1 mm in
diameter) and removed from the matched paraffin tissue blocks. Tissues
were then deparaffinized and processed as described above. After embedding
all tissues in epoxy resin, semi-thin sections were cut and stained with
alkaline toluidine blue for histologic analysis. The thin sections of the
selected blocks were stained with lead citrate and uranyl acetate and examined
by electron microscopy.
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