- Dear Jeff,
-
- While Bob Beck was still alive, he tried in vain to find
this presentation from Dr. Steven Kaali and Dr William Lyman
of the Albert Einstein College of Medicine in NYC detailing the discovery
of blood electrification. He was trying hard to find the original
presentation they made at the 1991 AIDS conference in Washington DC,
but he couldn't locate it, no matter how many libraries he visited. The
best he could do was to study the US patent that Kaali filed in 1992. A
couple of days ago, I was sent five photos taken of the updated 1996 report
found in an extremely obscure medical ournal. To the best of my knowledge,
this is the first time this report has ever been posted on the internet.
It explains just how easy it is to deactivate the HIV virus-- from the
horse's mouth. Bob Beck would have been thrilled.
-
- You'll have to go to this url to see the graphs.
-
- http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml
-
- For some reason I couldn't get them to paste in
-
- Regards, Ken
-
-
- Biocompatible Electric Current
-
- Attentuates HIV Infectivity
-
- From Ken Adachi, <http://educate-yourself.org/be/beckelectrifierinfo.shtml>
- Editor: This is an extremely important report for anyone
who is concerned about treating AIDS or HIV infection. Photos of the five
pages of this 1996 report which appeared in Surgical Overview, Surgical
Technology International V,were sent to me by a very sharp scientist and
humanitarian named Webster Kehr <<mailto:jammstone@gmail.com>jammstone@gmail.com>
on November 18, 2006. Webster deserves real credit for finding this published
report as much effort has been expended to suppress this Kaali and Lyman
1990 discovery at the Albert Einstein College of Medicine in New York City.
For example, Dr. Bob Beck found that the text of the March 1991 Washington
DC AIDS conference where Kalii and Lyman first presented their findings
publicly, were razor cut out of library copies of the published journal.
Bob was able to find only three brief news items immediately following
the March AIDS conference in
- <http://educate-yourself.org/cn/behoustonpost.shtml>
- The Houston Post (Mar 20, 1991), <http://educate-yourself.org/cn/besciencenews.shtml>Science
News (Mar. 30, 1991 ) and <http://educate-yourself.org/cn/belongevity.shtml>
- Longevity magazine: and then nothing.
-
- The Discovery of the Century to address the greatest
bio-engineered 'disease' in modern history, AIDS, was on the receiving
end of one of biggest media blackouts ever to be perpetrated on the American
public. How many people, to this day, still believe that AIDS is 'incurable'?
About 99.999% of the public I would guess.
-
- To make the report readable, I had to enlarge Webster's
photos using Photoshop so I could read it and then typed out the text of
the report. I've inserted the graphs from the photos of the report into
the typed version seen below. This report is a more refined presentation
than that provided by Dr. Steven Kaali in his 1992 U.S. patent application
(<http://patent.womplex.ibm.com/details?patent_number=5139684>
- #5,139,684 Kaali & Schwolsky 8-18-92)which
- <http://educate-yourself.org/be/bekaaliexperiment.shtml>
- I posted to www.educate-yourself.org in January of 2000
- (<http://educate-yourself.org/be/bekaaliexperiment.shtml>
- http://educate-yourself.org/be/bekaaliexperiment.shtml).
-
- This discovery by Kaali and Lyman in the Fall of 1990
was the centerpiece of Dr. Bob Beck's lectures on <http://educate-yourself.org/be/index.shtml>blood
electrification. Kaali and Lyman re-discovered something that Dr. Robert
O. Becker had also came upon in the 1970's and 80's in that direct current
applied at very low voltage, delivered in the 50-100 microampere range
effected amazing cellular response and achieved the de-activation of pathogenic
organisms.
-
- Kaali and Lyman patented an invasive procedure to insert
the DC micro currents. They opened up an artery and sewed in a tiny battery
driven circuit with two tiny electrodes within the artery itself. After
the battery ran out, they would remove it and insert a fresh unit in another
artery location. After 5 or 6 months, the patient showed greatly lowered
HIV viral loads and steadily recovered. Bob Beck, on the other hand, invented
a non-invasive method of inducing the micro currents electro-magnetically
by applying external electrodes to the wrists and used a bi-phasic square
wave of 3.92Hz to achieve the same thing as Kaali and Lyman with their
internal arrangement. Bob called it "blood electrification" and
his unit is called a "<http://educate-yourself.org/be/beckelectrifierinfo.shtml>blood
electrifier" (you can <http://educate-yourself.org/be/becknexusarticle1.shtml>make
your own from instructions passed out freely by Bob Beck or get a factory
made unit. <http://educate-yourself.org/contactus/>Contact me for
more information).
-
- An important consideration when applying DC (direct current)
voltage to the body involves the physics of electrolysis. Dr. Robert O.
Becker found that DC voltages higher than 1.1 volt caused sufficient electrolysis
action that the body became overwhelmed and produced electrolysis 'waste'
products in the region where the DC voltage was applied. Bob Beck got around
the electrolysis problem by using an AC (alternating current) voltage in
the form of a bi-phasic (two phase, positive and negative) square wave.
However, Dr. Hulda Clark is adamantly opposed to using any application
of negative voltage, whether as pure negative DC voltage or the negative
half of an AC waveform (including the negative half cycle of a bi-phasic
square wave). Hulda found that the slightest amount of negative voltage
will encourage the growth of pathogenic organisms;something we're trying
to avoid.
-
- For those who wish to experiment, you could insert a
solid state diode rectifier in series on the "hot" side of the
electrode wiring (the other electrode is at "ground" or zero
potential) of the Beck electrifier and have the anode of the diode connect
to the electrode itself. The diode rectifier will cut off the negative
excursion of the Beck bi-phasic electrifier and leave you with onlypositive
pulsing DC square waves. The square wave pulses are only "on"
for 50% of each cycle and "off" for the remaining 50%. The 50%
"off time" may prevent the electrolysis problem, but that's just
a theoretical conjecture on my part. I haven't done any experiments to
confirm this one way or the other. Another possible approach is to reduce
the pulsing square wave down to a very narrow duty cycle pulse of say 20%
. A third possibility is to switch over to a very sharp rise pulse. A possible
downside in using a narrow pulse is that it might not deliver the requisite
50-100 microampere current desired, however, it might do it -if only momentarily.
There's much food for thought here and an open invitation to anyone who
can still think outside the Establishment box, to get busy and see what
marvelous results you can obtain by following in the footsteps of both
Dr. Robert C. Beck and Dr. Robert O. Becker.
-
- A few notes on nomenclature used in this report:
-
- 1. "sup.2" refers to 'supra' (above) or raised
to the power of 2 (or squared); so 10cm sup.2 = 10centimeters squared.
Another example, "10 sup.5" equals 10 raised to the 5th power
(100,000).
- 2. "sub.2" refers to the number 2 appearing
below the letter in front of it as seen with H2O
- 3. "In vitro" means that the test was made
in a test tube or petri dish outside of the body.
- 4. "In vivo" refers to conditions inside the
body.
- 5. "uL" = micro liters
- 6. "uA" = micro amperes.
- 7. "p" = "parts" and "<"
= "greater than"
-
- In a few places, I couldn't determine the correct number
due to glare in the photo or a bad viewing angle so I had to place a question
mark in parentheses (?). If you want to see the reference noted, click
on the small number linked in parentheses and you'll go directly to the
reference note. To get back to the same place where you were reading, hit
the "back" button/arrow.
-
- Sincerely, .Ken Adachi]
-
- © Copyright 2006 Educate-Yourself.org All Rights
Reserved.
-
-
- By William D. Lyman, Ph.D and Steven G. Kaali, M.D, Albert
Einstein College of Medicine
- http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml
- Posted November 18, 2006
-
- Subject: Kaali and Lyman Paper
- From: Webster Kehr
- jammstone@gmail.com>
- Date: Sat, November 18, 2006 7:39 am
- To: Editor of www.educate-yourself.org
-
- Gentlemen:
-
- In 1990, the greatest discovery in the history of medicine
took place, the discovery of the cure for AIDS/HIV and very other microbe
caused disease on earth. They should have won the Nobel Prize, but their
discovery was crushed. However, the two medical doctors involved, Kaali
and Lyman, found ways to make their discovery public. They filed several
patents, as did several others. While their original paper is lost to the
world,.in 1996
- they quietly got it republished in a very obscure journal.
Their discovery was the basis for the Bob Beck Protocol. I have obtained
a copy of the
- article and am sending it to you in 5 different emails,
one email for each page. Please keep these 5 files in a permanent location
and let me know you
- got all 5 of them.
-
- Regards,
- Webster
-
- ****
- Biocompatible Electric Current Attentuates HIV Infectivity
-
- William D. Lyman, Ph.D., Professor
- Depart of Pathology
- Albert Einstein College of Medicine
- Bronx, New York
-
- Irwin R. Merkatz, M.D., Professor and Chairman
- Steven G. Kaali, M.D., F.A.C.O.G., Clinical Asociate
Professor
- Department of Ovstetrics and Gynecology
- Albert Einstein College of Medicine
- Bronx, New York
-
- Introduction
-
- The number of individuals infected by the human immunodeficiency
virus type-1 (HIV) continues to increase on a world wide basis. <http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#1>
- (1) A significant percentage, if not all, of these individuals
will eventually develop the acquired immunodeficiency syndromes (AIDS).<http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#2>
- (2) While horizontal transmission in the homosexual population
may be contained or decreasing,<http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#3>
- (3)heterosexual transmission and infection through contaminated
blood supplies continues to increase. <http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#4>
- (4) Additionally, vertical transmission from infected
females to their fetuses is also on the rise with a resultant increase
in the number of children with AIDS.<http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#5>
- (5) New strategies, therefore, must be devised in order
to limit more effectively the spread of this virus.
-
- In this regard, three principal approaches are currently
being investigated. In order to decrease susceptibility to the consequences
of infection, vaccines are being sought which will induce the production
of protective antibodies.
- <http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#6>
- (6) As treatment modalities, the use of soluble antagonists
t block the receptor for HIV is being studied <http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#7>
- (7) as are pharmacologic agents such as nucleic acid
analogues which can interfere with the transcription of viral genomic sequences.
<http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#8>
- (8) Each of these systems has virtues and limitations,
and to date none has proven completely efective.
-
- Because heat or light in combination with drugs and dyes
can inactivate viruses including HIV in vitro, <http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#9>
- (9) others have suggested the use of these forms of energy
to treat AIDs patients. The results of studies using heat have not been
peer reviewed and are therefore impossible to evaluate. The use of light
with drugs ("photopheresis")
- <http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#10>
- (10) appears to be efficacious, although this treatment
may be limited by drug toxicity and the potential long-term effects of
ultraviolet radiation on blood cell nucleic acids. Also, by its nature,
this last system may not be suitable for the treatment of tissue associated
virus. As a result of our interest in the use of electric current to alter
biological systems, we focused our investigations on the ability of direct
electrical current at biocompatible levels to alter the infectivity of
HIV for susceptible CD4 positive cells in vitro.
-
- MATERIAL AND METHODS
-
- Electrical Treatment of HIV
-
- The RF strain of HIV (AIDS Reagent Program) was cryopreserved
prior to treatment at -70 degrees C. For treatment, a sample of virus was
thawed and maintained on ice at 4 degrees C. Ten microliters (uL) of HIV
at a concentration of 10 sup.5 infectious particles per mL were placed
into a chamber which included a pair of platinum electrodes 1mm apart permanently
mounted into a well 1.56 mm in length and 8.32 mm in depth equal to 12.9uL
volume capacity. The chamber was connected to a power supply capable of
creating constant direct current. The viral aliquots were exposed to direct
currents ranging form 0 micro amperes (uA) for up to 12 minutes to 100UA
for up to 6 minutes. Intermediate currents of 25, 50, and 75 uA were used
to expose similar viral aliquots. Under these conditions, for example,
0, 50, and 100 uA represent 0, 3.85, and 7.7 uA/mm sup.2 current densities
respectively. The current was monitored throughout the experiment.
-
- A matrix of current and time employed is shown in Table
1.
-
- After the exposure of virus to electric current, the
contents of the chamber were removed and placed into sterile micro tubes.
Five uL of each sample were removed and diluted with 95 uL tissue culture
medium supplemented with 10% fetal calf serum (FCS) for subsequent assays.
-
- Syncytium-Formation Assay
-
- This assay was performed as previously described by Nara
et al. <http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#11>
- (11) Briefly, 10 sup.5 CEM-SS cells were dispensed into
poly-L-lysine coated microtiter wells. Thereafter, tenfold dilutions of
H9 cells incubated with the treated HIV samples were co-cultured in triplicate
for up to 4 days with the CEM-SS cells. Identical wells were prepared with
control uninfected and infected cells. The wells were examined for syncytium
formation at 2 and 3 days and quantified using an inverted microscope.
-
- Reverse Transcriptase Assay
-
- Uninfected H9 cells were pelleted at 1,000 rpm for 5
minutes at room temperature, the supernatant was decanted, and the cells
were resuspended in 100 uL treated viral sample. The cells were incubated
for up to 6 hours with the viral samples. At the end of the incubation
time,. the viral/cell suspensions were centrifuged at 1,000 rpm for 5 minutes
and the supernatant decanted. The cell pellet was then resuspended in 5mL
of RPMI, 10% FCS and placed into a T25 tissue culture flask and maintained
at 37 degrees C, 5% COsub.2 in a humidified chamber. At 2 day intervals
(beginning at day 2), 1 mL of the cell suspensions was removed from each
sample and centrifuged at 1,000 rpm for 5 minutes in order to pellet the
cells. The supernatant was subsequently centrifuged at 14,000 rpm for 15
minutes. the pellet was resuspended in suspension buffer and assayed using
standard methodology employing Mg++ as the divalent cation, poly (rA) oligo
d(T) 12-18 as template primer, and tritiated thymidine (sup.3H-TdR) which
comprise the reaction mixture. Known HIV positive and negative control
samples were included in each assay for reference. Thirty uL of the reaction
mixture were added to each 10 uL viral sample and incubated at 37 degrees
C for 60 min. Samples were then incubated with 1uL of cold quench solution
on ice for 15 minutes and filtered through a Millipore manifold. Chimneys
were rinsed first with wash solution and followed by cold 95% ethanol.
The filters were dried by vacuum and counted in scintillation fluid. Reverse
transcriptase activity is expressed as counts per minute (cpm) and is considered
positive only if cpm are at least five times greater than cpm obtained
with HIV-negative control samples.
-
- Biocompatibility of Electric Currents/Time
-
- To determine if the electric currents used were in a
biocompatible range of energy, uninfected H9 cells were exposed to distinct
currents for different amounts of time. The H9 cells were washed two times
in Hanks Balance Salt Solution (HBSS). Thereafter, the cells were resuspended
in RPMI, 10% FCS at a concentration of 10sup.(?) cells per mL. Ten uL of
the cell samples were placed into the reaction chamber. The cell samples
were then exposed to 0, 50, or 100 uA for 0, 3, or 6 minutes. At the end
of each test, the cell sample was removed from the chamber and approximately
10 uL of the sample was mixed with 90 uL of tyrpan blue. The number of
viable cells was determined by trypan blue exclusion using a hemocytometer
and light microscope. Results are expressed as percentage of viable cells
from the total of all cells. At least 200 cells per field were counted.
-
- Statistical Analysis
-
- Results of the syncytium-formation and reverse transcriptase
assays were tested for statistical significance by the Student's t test
and analyses of variance.
-
- RESULTS
-
- Syncytium-Formation Assay
-
- Using this index of HIV infectivity, it was determined
that exposing virus to direct electric current suppressed its capacity
to induce the formation of syncytia. Figure 1 shows a representative experiment
and Table 2 shows the group data for three separate experiments. As can
be noted in Figure 1, a statistically significant (p<0.001) reduction
in syncytium number was observed, and this reduction was dependent upon
the current applied to the viral isolate. At three different viral dilutions,
there were analogous results in that a total charge of 200 uA x min (25uA
for 8 minutes) reduced the number of syncytia from 50% to 65% while a charge
of 300 uA x min (50uA for 6 minutes, 75 uA for 4 minutes, or 100uA for
3 minutes) resulted in 90% reduction.
-
-
-
- Reverse Transcriptase Assays
-
- The direct electric currents to which HIV was exposed
also reduced reverse transcriptase activity. Five separate experiments
were conducted; a representative experiment is shown in Figure 2 and the
group data are included in Table 3. As can be seen in Figure 2, there was
a significant decrease in the amount of reverse transcriptase activity
after exposure of the virus to either 50 uA for 3 or 6 minutes . An equivalent
reduction in reverse transcriptase activity was also noted with exposure
to 100 uA for 3 minutes. and near ablation of reverse transcriptase activity
was seen with exposure of the viral isolate to 100uA for 6 minutesresulted
in a 94% reduction. An analysis of variance indicates that the decrease
in reverse transcriptase activity was statistically significant (p<0.001).
-
- Biocompatibility of Electric Currents/Time
-
- The ------(?) of a viability analysis using trypan blue
exclusion criteria applied to uninfected cells exposed to the different
currents and times used for these studies are shown inTable 4. The viability
of H9 cells, after exposure to 100uA for either 3 or 6 minutes, did not
show a significant decrease when compared to the 0 current control. After
maximum treatment at 100 uA for 6 minutes, cell viability was 93% . Interestingly,
in other preliminary experiments in which HIV-infected H9 cells were used,
the results show that at 100 uA there may have been a significant decrease
in the number of viable cells. That is, while an instantaneous pulse of
100 uA did not affect the viability of infected cells, a decrease in viability
was noted at 3 and 6 minutes of exposure to 100 uA. This decrease was time-dependent
in that exposure to 100 uA for 3 minutes resulted in a viability of 83%
while 100 uA for 6 miutes resulted in a viability of 80%. Although theses
data are provocative, they only represent a preliminary experiment and
require further investigation.
-
- With respect to the possibility that the electric current
was transduced into heat, the calculated rise in termperature within the
chamber was determined to be less than 1 degree C. In order to verify this,
a temperature microprobe was introduced into the chamber containing tissue
culture medium alone.
-
- Results of these studies are shown in Table 5. Similar
results were obtained when H9 cell-containing medium was placed in the
reaction chamber. The data indicate that for the currents and times used
for these experiments, there was no alternation in the temperature of the
chamber.
-
-
- DISCUSSION
-
- The results repored here demonstrate that HIV treated
with direct electric currents from 50 to 100 uA has a significantly reduced
infectivity for susceptible cells in vitro. This reduction of infectivity
correlates with the total electric charge passing through the chamber.
Although extrapolation of these data predicts that ablation of HIV infectivity
may be possible, and additional preliminary data support this prediction,
the expectation that some virions may still excape the electrical effect
cannot be discounted. Nevertheless, the therapeutic potential of electric
current may reside in its ability to lower the viral titer to subclinical
significance or in its incorporation into a strategy analogous to that
of other therapies in which repeated cycles of treatment eventually achieve
remission or cure.
-
- The data presented in this report are based on both quantitative
and quantal determinations of viral infectivity. Although the syncytium-formation
assay can be used to quantify the number of infectious viral particles,
this use with respect to HIV may be abridged because of the ability of
free fusigenic peptide (gp41) to induce syncytia by itself. Therefore,
while syncytia were observed at some dilutions of electrically treated
virus, this may simply represent the presence of soluble gp41 in the tissue
culture medium. We believe that the correlation between total charge and
reduction in syncytium number more adequately reflects the ability of direct
electric current to reduce HIV infectivity.
-
- This belief is also suported by the results of the reverse
transcriptase assays. Although a decrease in HIV reverse transcriptase
does not assure reduced infectiousness of this virus for susceptible cells,
we feel that, taken together with the syncytium-formation data, the results
indicate that significant attenuation of HIV infectivity is achieved by
treatment with direct electric currents.
-
- With respect to the biocompatibility of the electric
currents and toal charges reported here, two separate sets of evidence
are applicable. The first has to do with the results showing that, by trypan
blue exclusion, no significant cytotoxicity was induced in H9 cells by
any total charge tested. The other evidence is obtained from reports which
clearly indicate that the amount of electricity used for these experimetns
is significantly below presently used therapeutic electric curents which
are in the milliampere range. (<http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#12>12-
- <http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#16>16)
-
- Rather than negative effects, exposure of cells to electric
current may actually have positive consequences for resistance to infection
in that important cellular electrochemical changes correlate with enhancement
of specific enzymatic activities. In particular, a facilitation of succinate
dehydrogenase (SDH) and ATPase activity has been observed. <http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#12>
- (12, <http://educate-yourself.org/cn/LymanKaalibiocompatibleHIV1996report18nov06.shtml#15>15)
- Both of these enzymes are associated with the oxidative
capacity of the cell. Specifically, it has been suggested that an electrochemical
reaction occurs between mitochondrial membrane-bound H+ ATPase and ADP
leading to the formation of ATP.Therefore, exposure of cells to direct
electrric currrent may directly or indirectly increase energy resources
within a cell and facilitate cell metabolism. This, in turn, may actually
render a cell less susceptible to the effects of viral infection.
-
- In summary, the data presented here indicate that biocompatible
direct electric current significantly reduces the infectivity of HIV. Continuing
investigations are exploring the mechanisms through which this effect is
mediated. The initial focus of these experiments is centered on the potential
role which ionic and molecular species generated by electrolysis may have
on the virus. However, the complete mechanism by which direct electric
current attenuates HIV infectivity is undoubtedly far more complex than
simple electrolysis. Nonetheless, and independent of a complete understanding
of all of the mechanisms involved in the attenuation of HIV infectivity,
the present observations may serve as an initial step for the development
of new strategies to treat infection or prevent transmission of HIV through
the treatment of the blood supply.
-
- ACKNOWLEDGEMENT
-
- The authors wish to thank Mrs. Barbara Shea for her excellent
secretarial assistaince and Dr. Gabor Kemeny for important technical help.
(STI)
-
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-
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in rat skin. Clin Ortho Rel Res 1983;175:263-72.
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- 13. Frank C, Schachar N, Dittrich D, et al. Electromagnetic
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Med 1979;7:169-71.
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